id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315931-kc8gnj6z	Klempt, Petr	Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis	2020-09-29		.txt	text/plain	4812	224	49	During the first attempt, thirty libraries (including 4 positive and 2 negative controls) were prepared in three plexes (10 samples each, see Supplementary Table S1 ) employing NEBNext ® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) followed by capture-based workflow utilizing the Twist SARS-CoV-2 Research Panel (Twist Bioscience). Compare to NEB+TWIST the capture-based Illumina approach data showed in average a lower percentage of mapped reads (Supplementary Table S1 ), this consequence could be related to the lower specificity of Respiratory Oligo Viral Panel designed next to the SARS-CoV-2 also for other pathogens (see https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/ngsenrichment-coronavirus-app-note-1270-2020-002.pdf). Also, the viral load in a sample (corresponding to sample Ct value ≤ 23.29 or positive control value ≤ 25.84) showed to be the limiting factor in case of each workflow, samples with higher Ct value resulted in either poor genome coverage (NEB+Twist workflow and Illumina workflow) or in absence of expected library preparation product (Paragon workflow).	./cache/cord-315931-kc8gnj6z.txt	./txt/cord-315931-kc8gnj6z.txt