id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314947-fy1lqk00	WU, Xiao Dong	The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells	2004-10-17		.txt	text/plain	3908	211	60	title: The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. Furthermore, antibodies against S1, S2, N protein could detect their corresponding proteins from the lysates of SARS-CoV infected Vero E6 cells. To First, S2-specific mAb was directly used to detect native S2 fragment or full length of S protein in the lysate of SARS-CoV infected Vero E6 cells.	./cache/cord-314947-fy1lqk00.txt	./txt/cord-314947-fy1lqk00.txt