id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314687-kyj6etnc	Gunalan, Vithiagaran	A putative diacidic motif in the SARS-CoV ORF6 protein influences its subcellular localization and suppression of expression of co-transfected expression constructs	2011-10-25		.txt	text/plain	4918	222	45	Following this, a mammalian expression plasmid pXJ3'-ORF6 was transfected into Vero E6 cells and Confocal microscopy showed that the ORF6 protein localized to a similar population of intracellular vesicles. These alanine substitution mutants were cloned into the same vector as the wildtype ORF6 gene and titrated against the nsp8 gene, by co-transfection of Vero E6 cells with plasmids encoding for myc-nsp8 and either 2 g pXJ3' ORF6 Figure 3 The ORF6 protein exerts a transcriptional effect on nsp8 expression. This indicated that the reduction in the suppression of the expression of co-transfected myc-nsp8 by ORF6A53-56 was significant, and therefore that the putative diacidic motif defined by amino acids 53-56 has a role to play in this ability of the ORF6 protein. This indicated that the putative diacidic motif from amino acids 53-56, in addition to being involved in the suppression of the expression of co-transfected myc-nsp8, is also involved in the subcellular localization of the ORF6 protein and therefore these 2 phenomena may be linked.	./cache/cord-314687-kyj6etnc.txt	./txt/cord-314687-kyj6etnc.txt