id author title date pages extension mime words sentences flesch summary cache txt cord-313265-lff5cajm Conway, Michael J. Identification of coronavirus sequences in carp cDNA from Wuhan, China 2020-03-16 .txt text/plain 918 61 61 Severe acute respiratory syndrome (SARS)‐like coronavirus sequences were identified in two separate complementary DNA (cDNA) pools. SARS-like virus sequences that were highly homologous to SARS-CoV-2 were identified in two separate complementary DNA (cDNA) pools. Tblastn analysis using this sequence identified two cDNA clones that were highly homologous to SARS-like coronaviruses. The first cDNA pool was made from Carassius auratus (crucian carp) blastulae embryonic cell line and contained a sequence of 152 amino acids that covered 2% of the SARS-CoV-2 genome and was 93.42% The second cDNA pool was made from Ctenopharyngodon idella (grass carp) head kidney and contained a sequence of 88 amino acids that covered 1% of the SARS-CoV-2 genome and was 93.18% identical. Protein and nucleic acid alignments of each cDNA clone were performed to compare with the most related coronavirus sequences (Figures 1 and 2) . ./cache/cord-313265-lff5cajm.txt ./txt/cord-313265-lff5cajm.txt