id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312633-cks6aij2	Cotten, Matthew	Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus	2013-05-17		.txt	text/plain	4063	229	55	Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events. We describe a strategy for rapidly designing the primers necessary for reverse transcription and cDNA amplification of such diverse RNA viruses and report the full-genome determination of the novel CoV directly from patient sputum using next-generation short-read sequencing. A map of the primer mapping positions Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus and the predicted PCR products using EMC/2012 as the target is shown in Figure 1 , panel A. A ML phylogenetic tree inferred from the whole genome alignment indicated that the 3 novel human beta-CoVs sequences (England1, England/Qatar/2012, and EMC/2012) clustered closely, forming a monophyletic lineage that falls into group 2c (Figure 3 , panel A, Appendix, wwwnc.cdc.gov/EID/article/19/5/13-0057-F3.htm).	./cache/cord-312633-cks6aij2.txt	./txt/cord-312633-cks6aij2.txt