id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311835-dmqfij6j	Siu, Kam-Leung	Comparative analysis of the activation of unfolded protein response by spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus HKU1	2014-01-13		.txt	text/plain	4917	306	55	title: Comparative analysis of the activation of unfolded protein response by spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus HKU1 We and others have previously shown that severe acute respiratory syndrome coronavirus (SARS-CoV) and mouse hepatitis virus (MHV) spike (S) proteins induce ER stress and activate cellular unfolded protein response (UPR) in the ER [3] [4] [5] . We compared the UPR-activating activity of SARS-CoV and HCoV-HKU1 S proteins in terms of their influence on the expression of UPR effectors Grp78, Grp94, CHOP and PERK. To analyze further whether PERK activity is required for transcriptional activation of Grp78 and Grp94 promoters by SARS-CoV and HCoV-HKU1 S proteins, we made use of a dominant negative (DN) mutant of PERK which constitutively inhibits PERK kinase activity [48] . To determine whether the UPR-activating property of SARS-CoV S protein is mediated by S1 (amino acids 1-770) or S2 (amino acids 771-1255) subunit, we expressed them in 293FT cells ( Figure 6A, lanes 2 and 3) .	./cache/cord-311835-dmqfij6j.txt	./txt/cord-311835-dmqfij6j.txt