id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311599-m400cal3	Lehmann, Christian	A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses	2008-05-31		.txt	text/plain	5031	289	50	Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). Here, we describe the establishment of a line immunoassay for rapid and simultaneous detection of antibodies directed against the 5 known HCoVs (229E, NL63, OC43, HKU1, and SARS) in human sera based on recombinant viral N proteins. Table 2 Reactions of clinically defined sera with HCoV nucleocapsids In sera taken during acute phase of HCoV infection, no OC43-or 229E-specific antibodies could be detected, whereas samples collected after convalescence strongly reacted with the corresponding antigens. Consistently, all available human sera sampled from convalescent patients (tested positive for HCoVs 229E, OC43, or SARS-CoV by microneutralization experiments, Western blotting, ELISA, or IF) reacted with their respective antigens as expected, indicating an excellent sensitivity of our line immunoassay.	./cache/cord-311599-m400cal3.txt	./txt/cord-311599-m400cal3.txt