id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310291-z79x349o	Holland, LaRinda A.	An 81-Nucleotide Deletion in SARS-CoV-2 ORF7a Identified from Sentinel Surveillance in Arizona (January to March 2020)	2020-07-01		.txt	text/plain	1052	67	52	title: An 81-Nucleotide Deletion in SARS-CoV-2 ORF7a Identified from Sentinel Surveillance in Arizona (January to March 2020) Here, we report on early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sentinel surveillance in Tempe, Arizona. Genomic characterization identified an isolate encoding a 27-amino-acid in-frame deletion in accessory protein ORF7a, the ortholog of SARS-CoV immune antagonist ORF7a/X4. In anticipation of COVID-19 spreading in Arizona, we initiated a surveillance effort for the local emergence of SARS-CoV-2 starting 24 January 2020. To understand the evolutionary relationships and characterize the SARS-CoV-2 genomes, we performed next-generation sequencing (NGS; Illumina NextSeq, 2ϫ76) directly on specimen RNA, thereby avoiding cell culture passage and potentially associated mutations. We found that the SARS-CoV-2 AZ-ASU2923 genome has an 81-nucleotide (nt) deletion in the ORF7a gene, resulting in a 27-amino-acid in-frame deletion (Fig. 2B) . Severe acute respiratory syndrome coronavirus gene 7 products contribute to virus-induced apoptosis	./cache/cord-310291-z79x349o.txt	./txt/cord-310291-z79x349o.txt