id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308451-pmwmfl3w	Chiang, Shu-Fen	SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells	2010-07-27		.txt	text/plain	6273	345	51	In this report, we showed that spike protein of SARS virus could induce phenotypic conversion of human B cells, either from peripheral blood or B lymphoma cells, to macrophage-like cells that were steadily losing the B-cell marker CD19 and in turn expressing the macrophage-specific marker Mac-1. In conclusion, our results suggest that conversion of B cells to macrophage-like cells, similar to a pathophysiological response, could be mediated by a devastating viral ligand, in particular spike protein of SARS virus, or in combination with severe local hypoxia, which is a condition often observed in afflicted lungs of SARS patients. Our results show that spike protein of SARS displayed on recombinant baculovirus or prolonged exposure to hypoxia can trigger the conversion of peripheral B cells and B lymphoma cells into Mac-1 positive macrophage-like cells. As determined by microarray analysis, expression of CD86 (B7-2) gene was rapidly induced in B lymphoma cells within 12 h of SSDRB treatment (Fig. 4B) .	./cache/cord-308451-pmwmfl3w.txt	./txt/cord-308451-pmwmfl3w.txt