id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303745-wx3udkee	Martinez-Fleta, P.	SARS-Cov-2 cysteine-like protease (Mpro) is immunogenic and can be detected in serum and saliva of COVID-19-seropositive individuals	2020-07-18		.txt	text/plain	5031	328	57	Using ELISA tests, coating several SARS-CoV-2 proteins produced in vitro, we describe that COVID-19 patients make high titre IgG, IgM and IgA antibody responses to the Cys-like protease from SARS-CoV-2, also known as 3CLpro or Mpro, and it can be used to identify individuals with positive serology against the coronavirus. Since this study evaluated, for the first time, whether coronavirus-infected individuals could 121 generate an antibody response against the Cys-like protease, MPro, other SARS-CoV-2 122 proteins, commonly used in serology tests, were produced, for comparison. 1101 individuals with high specificity and sensitivity 153 A cohort of 36 COVID-19 patients (PCR+) and 33 healthy donors was recruited at La Princesa 154 University Hospital, Madrid (Table 1 ) and ELISA assays were performed to detect Mpro-, as 155 well as RBD-and NP-, specific antibodies of the IgG, IgA and IgM subclasses in sera ( Figure 156 3).	./cache/cord-303745-wx3udkee.txt	./txt/cord-303745-wx3udkee.txt