id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302358-vou46eie	Fomsgaard, Anna S	An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage	2020-03-30		.txt	text/plain	1720	114	58	With a detection sensitivity of 97.4% (95% CI=86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 minutes at 98°C prior to the RT-qPCR reaction. Analysis of SARS-CoV-2 positive and negative oropharyngeal swaps using the SensiFAST TM kit showed a 97.4% sensitivity, 100% specificity and 98.3% accuracy when samples were heat-processed for 5 min. Analysis of the clinical samples using the RealStar® SARS-CoV-2 RT-PCR kit showed significant inhibition of the RT-qPCR reaction (Figure 1 ) except when the MagNA Pure and QIACube purified samples were used. Moreover, this positive result could not be confirmed by any other NA purification method or SARS-CoV-2 specific RT-qPCR assay and therefore we cannot confirm if this patient sample is truly positive for COVID-19 using the QIACube purification system or false negative using the MagNA Pure system.	./cache/cord-302358-vou46eie.txt	./txt/cord-302358-vou46eie.txt