id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301227-ica5x0r1	Sun, Yi-Sheng	A SARS-CoV-2 variant with the 12-bp deletion at E gene	2020-10-29		.txt	text/plain	4410	236	65	In this study, by using plaque purification, we purified two SARS-CoV-2 virus strains from the same specimen, one named F8 containing a 12-bp deletion in the E gene and the other named 8X containing the wild-type E gene. In this paper, we isolated two SARS-CoV-2 strains with both wild-type and mutant E genes from the same sample using plaque purification. To explore whether other clinical samples contained a similar SARS-CoV-2 strain lacking a 12-bp sequence in the E gene, we used RT-PCR to detect the mutant E gene specifically. Our results indicated that both the F8 and 8X strains can infect Vero cells, however, the S protein content of the F8 viral culture was higher than that of 8X. In conclusion, we report an E gene mutant and a wild type SARS-CoV-2 strain isolated from the same clinical sample by plaque purification. In conclusion, we report an E gene mutant and a wild type SARS-CoV-2 strain isolated from the same clinical sample by plaque purification.	./cache/cord-301227-ica5x0r1.txt	./txt/cord-301227-ica5x0r1.txt