id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300784-4jeaqqn9	Ma, Huan	COVID-19 diagnosis and study of serum SARS-CoV-2 specific IgA, IgM and IgG by a quantitative and sensitive immunoassay	2020-04-22		.txt	text/plain	3493	203	56	Here, we aimed to develop a more quantitative and sensitive serological test for COVID-19 diagnosis, monitoring and clinical investigation, based on the detection of antigen-specific IgA as well as IgM and IgG in blood in response to SARS-CoV-2 infection. Methods In this investigation, we report the development of a set of validated diagnostic kits for detecting serum IgA, IgM, and IgG specific to SARS-CoV-2 nucleocapsid protein (NP) and receptor-binding domain (RBD) of the spike protein by chemi-luminescence immuno-analysis. . https://doi.org/10.1101/2020.04.17.20064907 doi: medRxiv preprint Based on the clinical RT-qPCR diagnosis results of SARS-CoV-2 infection, receiver operating 208 characteristic (ROC) analysis was conducted using MedCalc software to determine the optimal cut-off 209 value (criterion) and evaluate the diagnostic value of NP-or RBD-specific IgA, IgM, and IgG kits. Nonetheless, we showed that our serological kits based on SARS-CoV-2 spike 322 protein RBD as an immobilized antigen provide a high sensitivity and specificity for detecting IgA, IgM, 323 and IgG in a quantitative manner.	./cache/cord-300784-4jeaqqn9.txt	./txt/cord-300784-4jeaqqn9.txt