id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300040-rvrp5zvv	Dutta, Noton Kumar	Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine	2008-06-15		.txt	text/plain	4681	255	54	We constructed eukaryotic expression plasmid encoding N [(N1 (nucleotide: 1-1269), N2 (nucleotide: 1-327), and N3 (nucleotide: 328-1296)) gene fragments of the SARS-CoV and compared their individual potential immune responses in BALB/c mice for use in the development of SARS vaccine candidates. In this report, we detected SARS-Cov N1 and N3 protein-specific immune response induced by pVAX-N1 and N3 DNA vaccination, respectively, and found significantly high titres of specific antibody and specific cell mediated immunity compared to control. These results indicate that N protein, which naturally exists in virus particles after binding of viral RNA, was able to induce strong humoral and cellular immune responses when induced by DNA vaccine, and it might be a prospective candidate gene for development of SARS-CoV vaccine. showed that expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization. The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization	./cache/cord-300040-rvrp5zvv.txt	./txt/cord-300040-rvrp5zvv.txt