id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299480-mehwd0dk	Liu, Zheng-Xue	Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library	2005-04-08		.txt	text/plain	4261	225	62	Abstract To develop early diagnostic reagents, effective vaccines, and even drugs against SARS-associated coronavirus (SARS-CoV), the human single fold single-chain antibody fragments, (scFv) libraries I+J (Tomlinson I+J) were used to identify novel scFvs, which can specifically bind to SARS-CoV. Interestingly, two scFvs (B5 and B9) exhibited higher binding specificity to SARS-CoV with the OD450 value 0.608 and 0.545, respectively, and their coding sequences shared the identical sequence composed of VH gene (351bp) and VL gene (327bp), so the two scFvs were uniformly named as SA59B and chosen for further analysis. Hence, single-chain antibody fragments specific against SARS-CoV from phage-displayed antibody library have potential for exploitation as diagnostic or even antiviral therapeutic reagents. The ELISA plates coated with purified SARS-CoV lysate, positive and negative sera were provided by Military Medical Science Academic and HuaDa Gene Company (Beijing, China). The panning procedure was performed on a 96-well flexible assay plate coated with purified SARS-CoV lysate, which was blocked directly with MPBS (PBS containing 4% skimmed milk powder) at room temperature for 2 h.	./cache/cord-299480-mehwd0dk.txt	./txt/cord-299480-mehwd0dk.txt