id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299105-3ivzmiqn	Cheng, Yi‐Qiang	Deciphering the Biosynthetic Codes for the Potent Anti‐SARS‐CoV Cyclodepsipeptide Valinomycin in Streptomyces tsusimaensis ATCC 15141	2006-03-01		.txt	text/plain	3774	221	52	Sequence analysis of the NRPS system reveals four distinctive modules, two of which contain unusual domain organizations that are presumably involved in the generation of biosynthetic precursors d‐α‐hydroxyisovaleric acid and l‐lactic acid. Aimed at generating analogues by metabolic engineering, the valinomycin biosynthetic gene cluster has been cloned from Streptomyces tsusimaensis ATCC 15141. Aimed at generating analogues by metabolic engineering, the valinomycin biosynthetic gene cluster has been cloned from Streptomyces tsusimaensis ATCC 15141. Sequence analysis of the NRPS system reveals four distinctive modules, two of which contain unusual domain organizations that are presumably involved in the generation of biosynthetic precursors D-a-hydroxyisovaleric acid and L-lactic acid. The respective adenylation domains in these two modules contain novel substrate-specificity-conferring codes that might specify for a class of hydroxyl acids for the biosynthesis of the depsipeptide natural products. The key element of this approach is to sequence a certain number of random genomic clones and to identify candidate gene(s) involved in natural product biosynthesis.	./cache/cord-299105-3ivzmiqn.txt	./txt/cord-299105-3ivzmiqn.txt