id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298535-wmxlu3l1	Agnihothram, Sudhakar	Evaluation of Serologic and Antigenic Relationships Between Middle Eastern Respiratory Syndrome Coronavirus and Other Coronaviruses to Develop Vaccine Platforms for the Rapid Response to Emerging Coronaviruses	2013-11-18		.txt	text/plain	5036	235	43	In this article, we use alphavirus replicon vaccine vectors to express a panel of recombinant S and N proteins from distantly related alphacoronaviruses and betacoronaviruses, including MERS-CoV and other subgroup 2c CoVs. Using mouse polyclonal antisera and recombinant proteins, we compare the cross-reactivity and neutralization titers of these antisera between distantly related human and bat CoVs. Our results indicate that the S glycoprotein but not the N protein is the major determinant of the neutralizing antibody response to MERS-CoV; that the N proteins of CoVs only cross-react within but not between subgroups; that little if any cross-neutralization or cross-reactivity exists between the S proteins of CoVs within subgroup 2c or any other subgroup; and that cross-neutralization and cross-reactive patterns were validated with the convalescent-phase serum sample from a patient infected with MERS-CoV Hu/England-N1/2012 and a donor panel of human antisera against 3 different HCoVs. Our approach provides critical reagents, antisera, and recombinant virus vaccines that allow for rapid diagnosis of and intervention against MERS-CoV and other zoonotic CoVs that emerge in the future.	./cache/cord-298535-wmxlu3l1.txt	./txt/cord-298535-wmxlu3l1.txt