id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298406-7wfdwou8	Sun, Haifang	Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus	2003-12-31		.txt	text/plain	2678	161	60	title: Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus To facilitate the studies regarding the functions and structures of SARS_3CLpro, in this report the synthetic genes encoding 3CLpro of SARS_CoV were assembled, and the plasmid was constructed using pQE30 as vector and expressed in Escherichia coli M15 cells. In our previous work [24, 25] , we reported a 3D model of SARS_3CL pro and its inhibitor design by virtual screening, as well as the cloning, expression, and purification of the E protein of SARS_CoV. In this article, we would like to present the results describing the molecular cloning, expression and purification of 3CL pro of SARS_CoV, and the preliminary study on its mass spectrometric characterization is also reported. Expression and purification of SARS_3CL pro Escherichia coli M15 cells transformed with the plasmid pQE30-SARS_3CL pro were grown in 100 ml LB medium containing ampicillin (100 mg/L) and kanamycin (25 mg/L) at 37°C overnight and then inoculated into 1 L LB supplemented with both the antibiotics.	./cache/cord-298406-7wfdwou8.txt	./txt/cord-298406-7wfdwou8.txt