id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298098-4dfqlebp	Xu, Ruodan	Construction of SARS-CoV-2 Virus-Like Particles by Mammalian Expression System	2020-07-30		.txt	text/plain	3326	173	53	In the current study, using mammalian expression system, which is advantageous in maintaining correct protein glycosylation patterns, we efficiently constructed SARS-CoV-2 VLPs. We showed that among four SARS-CoV-2 structural proteins, expression of membrane protein (M) and small envelope protein (E) are essential for efficient formation and release of SARS-CoV-2 VLPs. Moreover, the corona-like structure presented in SARS-CoV-2 VLPs from Vero E6 cells is more stable and unified, as compared to those from HEK-293T cells. Construction of SARS-CoV-2 VLPs was performed by co-transfecting cells with S, M, E, and N with molar ratio at 8:6:8:3 as shown in Figure 1 . To get better understanding of the secretory features of four SARS-CoV-2 structural proteins, we first transfected HEK-293T and Vero E6 cells with vectors expressing S, M, E, or N, respectively. In the current study, we described the efficient construction of SARS-CoV-2 VLPs by plasmid-driven transfection of viral structural proteins in mammalian cells.	./cache/cord-298098-4dfqlebp.txt	./txt/cord-298098-4dfqlebp.txt