id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-297324-me5ff1pb	Zeng, Rong	Characterization of the 3a Protein of SARS-associated Coronavirus in Infected Vero E6 Cells and SARS Patients()	2004-07-30		.txt	text/plain	4579	223	57	Analysis of the genomic organization of SARS-CoV showed that a gene locus containing ORF3a and 3b located between the S and E genes, 6 -8 is frequently found in different members of the coronavirus family (see Figure 4A ). According to the genomic sequence information for SARS-CoV, ORF 3a encoded a putative 31 kDa protein containing 274 amino acid residues. 6, 7 In order to confirm the result by shot-gun assay, the collected cytosol of SARS-CoV-infected Vero E6 cells was separated with SDS-PAGE, and then the gel slice around the 31 kDa region was excised and analyzed by capillary liquid chromatography electrospray tandem mass spectrometry (mLC-ESI-MS/MS). Interestingly, comparison with S protein sequences of the coronavirus family also shows a cysteinerich region overlapping the junction of the membrane-spanning domain and the cytoplasmic tail, which is a conserved motif of spike proteins in all analyzed coronaviruses (see Figure 3 of Marra et al.	./cache/cord-297324-me5ff1pb.txt	./txt/cord-297324-me5ff1pb.txt