id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296672-i267t23m	Wang, Shui-Mei	Severe acute respiratory syndrome coronavirus nucleocapsid protein confers ability to efficiently produce virus-like particles when substituted for the human immunodeficiency virus nucleocapsid domain	2008-07-01		.txt	text/plain	5504	300	52	We replaced the HIV-1 nucleocapsid (NC) domain with different N-coding sequences to test SARS-CoV nucleocapsid (N) self-interaction capacity, and determined the capabilities of each chimera to direct virus-like particle (VLP) assembly. Each mutant was transiently transfected into 293T cells and subjected to Western immunoblotting to determine its ability to assemble and release VLPs. As shown in Fig. 2b , chimeric proteins with predicted molecular weights corresponding to Pr55 gag containing a SARS-CoV N coding sequence insertion in the NC region were readily detected. The above results suggest that HIV-1 Gag containing a large sequence of approximately 200-400 residues inserted into the NC region is still capable of assembling and releasing VLPs. Since the cultured 293T transfectant supernatant was centrifuged through 20% sucrose cushions for 40 min, we believe the recovered viral proteins in the pelleted medium are virus-associated.	./cache/cord-296672-i267t23m.txt	./txt/cord-296672-i267t23m.txt