id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291991-on70zzn0	Jaimes, Javier A.	Proteolytic cleavage of the SARS-CoV-2 spike protein and the role of the novel S1/S2 site	2020-05-28		.txt	text/plain	1700	93	58	Here we provide context and clarify the role of the novel SARS-CoV-2 S1/S2 cleavage site in virus 90 emergence and infection, and perform a direct assessment of the proteases cleaving this site by use of 91 biochemical assays. To directly address the proteases cleaving the SARS-CoV-2 S1/S2 site, we used a biochemical peptide 95 cleavage assay (Jaimes et al., 2019), which was previously used to screen emerging influenza viruses 96 (Straus and Whittaker, 2017) . The comparative data with SARS-CoV S1/S2 site reveals that the acquisition of the 4 109 amino acid insert distinctively broadens the activating protease repertoire of the SARS-CoV-2 S1/S2 110 cleavage site to all major classes of proteolytic enzymes known to potentially activate coronavirus S 111 proteins. Activation of the SARS coronavirus spike 195 protein via sequential proteolytic cleavage at two distinct sites A novel bat coronavirus closely related to SARS-CoV-2 contains natural insertions at 258 the S1/S2 cleavage site of the spike protein	./cache/cord-291991-on70zzn0.txt	./txt/cord-291991-on70zzn0.txt