id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291790-z5rwznmv	Li, Qianqian	The impact of mutations in SARS-CoV-2 spike on viral infectivity and antigenicity	2020-07-17		.txt	text/plain	4884	362	66	We first tested the infectivity of 106 pseudotyped viruses (80 natural variants and 26 129 glycosylation mutants) in 293T-hACE2 cells, where a difference by 4 -fold in RLU compared 130 with the reference Wuhan-1 strain (GenBank: MN908947) was deemed as being significant 131 ( Figure S1 ). Notably, some RBD variants such as A475V 283 and F490L have been confirmed to have decreased sensitivity to both human sera and multiple 284 neutralizing mAbs. A475V reduced the sensitivity to 6 mAbs out of the 13 mAb used in this study, 285 while F490L reduced the sensitivity to neutralization by 3 mAbs. It is possible that antibodies in 286 14 convalescent sera are able to neutralize these critical epitopes targeted by these mAbs that are 287 known to disrupt the binding of the S protein to hACE2 receptor (Ju et Serial dilutions of mAb preparations were pre-incubated with the pseudotyped viruses at 37°C for 355 one hour before they were added to Huh-7 cells.	./cache/cord-291790-z5rwznmv.txt	./txt/cord-291790-z5rwznmv.txt