id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287289-zgehbwve	Schmidt, M.	FACT- Frankfurt adjusted COVID-19 testing- a novel method enables high-throughput SARS-CoV-2 screening without loss of sensitivity	2020-05-01		.txt	text/plain	2381	154	60	title: FACTFrankfurt adjusted COVID-19 testinga novel method enables high-throughput SARS-CoV-2 screening without loss of sensitivity We applied a novel protocol to NAT testing of respiratory swabs for SARS-CoV-2: First, to evaluate for suitability of different mini-pool sizes, swabs were contaminated with a defined SARS-CoV-2 virus concentration of 1x10 4 copies/ml, and then placed in a series of 10 tubes with lysis buffer for 5 minutes each. P-value for individuals sample and mini pool NAT was 0.299 and 0.354 for the ORF region and E-gene, respectively, which we consider not statistically significant. Ct-values did not differ significantly between mini-pool and the single sample testing (p-value for the ORF region and E gene were 0.44 and 0.46, respectively) (table 4). Here, we present an alternate protocol (FACT) that is based on incubation of a respiratory swab first in a single sample tube, and then again in a mini-pool tube.	./cache/cord-287289-zgehbwve.txt	./txt/cord-287289-zgehbwve.txt