id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284091-1dj4yxkz	Duart, Gerard	SARS-CoV-2 envelope protein topology in eukaryotic membranes	2020-09-09		.txt	text/plain	2892	155	45	In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Computer-assisted analysis of the SARS-CoV-2 E protein amino acid sequence using seven popular prediction methods showed that all membrane protein prediction algorithms except MEMSAT-SVM suggested the presence of one transmembrane (TM) segment located roughly around amino acids 12 to 39 (table 1) , which is not predicted as a cleavable signal sequence according to SignalP-5.0 [7] . Since multiple topologies have been reported for previous coronavirus E proteins [13] [14] [15] [16] [17] , SARS-CoV-2 E protein insertion into the microsomal membranes in two opposite orientations cannot be discounted, but according to our data being dominant an Nt lum /Ct cyt orientation. As shown in figure 2c, E protein (NST) was efficiently glycosylated when microsomal membranes were added to the translation mixture cotranslationally (lane 4). Biochemical evidence for the presence of mixed membrane topologies of the severe acute respiratory syndrome coronavirus envelope protein expressed in mammalian cells	./cache/cord-284091-1dj4yxkz.txt	./txt/cord-284091-1dj4yxkz.txt