id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283786-d65njv7b	Toptan, Tuna	Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs	2020-06-20		.txt	text/plain	5258	235	53	The alignment of over 4300 full-length SARS-CoV2 genomes including FFM1-7 isolates revealed single nucleotide polymorphisms (SNPs) in 13 different positions for RdRP (total 0.33%) and eight positions for the M-gene (total 0.61%) within the primer/probe binding sites ( Figure S3 , Table S4 ). The alignment of over 4300 full-length SARS-CoV2 genomes including FFM1-7 isolates revealed single nucleotide polymorphisms (SNPs) in 13 different positions for RdRP (total 0.33%) and eight positions for the M-gene (total 0.61%) within the primer/probe binding sites ( Figure S3 , Table S4 ). Therefore, we additionally compared the performance of two research kits (New England Biolabs, Ipswich, MA, USA) and one In conclusion, our M-gene-based qRT-PCR detection of SARS-CoV-2 RNA was at least as specific as the RdRP PCR recommended for confirmation by the WHO but showed a significantly higher sensitivity.	./cache/cord-283786-d65njv7b.txt	./txt/cord-283786-d65njv7b.txt