id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283249-pk5sc2ca	Yoshida, Wataru	Homogeneous DNA sensing using enzyme-inhibiting DNA aptamers	2006-09-15		.txt	text/plain	5356	235	56	The structural change of the enzyme-inhibiting aptamer site induces a change in the inhibitory activity of the AES, which enables us to detect a target molecule by measuring the enzymatic activity of the whole aptameric complex in a homogeneous solution without bound/free separation. The stem-and-loop structure bearing the probe DNA sequence was inserted into the 3 0 -end T-T loop of the G-quartet structure of the 31-mer thrombin-inhibiting aptamer. We also inserted two additional T bases between the thrombin-inhibiting aptamer and the stem-and-loop structure in all AESs except AES 1, since the diameter of the DNA double helix is different from the distance between two Gs of the G-quartet structure (approximately 17 and 20 Å , respectively). For the measurement of the CD spectra of AES SARS 1, a stem-and-loop structure to be inserted into the 31-mer thrombin-inhibiting aptamer on AES SARS 1 was synthesized.	./cache/cord-283249-pk5sc2ca.txt	./txt/cord-283249-pk5sc2ca.txt