id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282895-85if4mnu	Xiao, Xiaodong	The SARS-CoV S glycoprotein: expression and functional characterization	2003-12-26		.txt	text/plain	3819	182	54	Fragments containing the N-terminal amino acid residues 17–537 and 272–537 but not 17–276 bound specifically to Vero E6 cells and purified soluble receptor, ACE2, recently identified by M. Here we report cloning, expression, and characterization of the SARS-CoV fulllength S glycoprotein and various soluble fragments, demonstration of its fusogenic function at neutral pH, development of a quantitative cell fusion reporter gene-based assay, and localization of the RBD in the N-terminal 303-537 residues. We have not observed measurable cytopathic effects in cells transfected with any of the constructs we developed (data not shown) indicating the possibility that the full-length and soluble fragments of the S glycoprotein may not have direct cytopathic effects. In an attempt to localize the receptor-binding domain (RBD) of the S glycoprotein prior to the identification of the SARS-CoV receptor, we developed an assay based on the binding of various soluble fragments to receptor expressing Vero E6 cells.	./cache/cord-282895-85if4mnu.txt	./txt/cord-282895-85if4mnu.txt