id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282821-qvtvpnrr	Thijsen, Steven	Elevated nucleoprotein-induced interferon-γ release in COVID-19 patients detected in a SARS-CoV-2 enzyme-linked immunosorbent spot assay	2020-06-12		.txt	text/plain	784	45	58	(2) (3) (4) The objective of the present study was to determine the functional T-cell responses to SARS-CoV-2 antigens (mosaic surface protein and nucleoprotein), by using an enzyme-linked immunosorbent spot (ELISpot) interferon-γ release assay, in patients with RT-PCR confirmed COVID-19 (n=27) and healthy controls (n=16). Our results show that the SARS-CoV-2-specific T-cell response measured in the ELISpot versus the dps induced by the mosaic surface protein and the nucleoprotein showed different patterns. In all but one of the 27 COVID-19 cases the T-cell response against the mosaic surface protein was absent or weak, as shown by the ELISpot results which were lower than 20 spot forming cells (SFC). In contrast, the Tcell response against the nucleoprotein measured by the ELISpot assay was elevated (10-150 SFC) in 12 of 19 patients (63%) that were sampled at ≥14 dps ( Fig. 1b) .	./cache/cord-282821-qvtvpnrr.txt	./txt/cord-282821-qvtvpnrr.txt