id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281860-zjvrohgg	Peng, Jing	Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2	2020-09-18		.txt	text/plain	2493	122	51	Moreover, sputum pretreated with saline, NALC, PK or DTT showed higher detection rates of SARS-CoV-2 as compared to pharyngeal swabs. To address this, we treated clinical sputum specimens with four commonly used reagents-saline, NALC, PK, and DTT, prior to NA extraction, and compared their performance in diagnosing COVID-19 in real practice. With the sputum samples collected from the 47 patients having non-COVID-19 diseases, no amplification curves were observed for either the ORF1ab or N gene under any treatment conditions (saline, NALC, PK, and DTT), suggesting no SARS-CoV-2 in these samples. According to the positive criteria described in the methods section, pretreatment of sputum samples with NALC, PK, and DTT increased the detection of SARS-CoV-2 + cases to 85.7% (18/21), 95.2% (20/21), and 95.2% (20/21), respectively, as compared to the 52.4% (11/21) obtained with saline pretreated sputum (see Table 1 ).	./cache/cord-281860-zjvrohgg.txt	./txt/cord-281860-zjvrohgg.txt