id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279476-h7zi82a8	Wang, Xueliang	Limits of Detection of Six Approved RT–PCR Kits for the Novel SARS-coronavirus-2 (SARS-CoV-2)	2020-04-13		.txt	text/plain	596	35	57	title: Limits of Detection of Six Approved RT–PCR Kits for the Novel SARS-coronavirus-2 (SARS-CoV-2) To cope with the COVID-19 epidemic, the China National Medical Products Administration (NMPA) approved six RT-PCR kits for SARS-CoV-2, and some of which subsequently received CE marking. To verify its applicability, the viral RNA was tested with the six kits provided by Shanghai Liferiver Bio-tech Co., Ltd, Wuhan Huada Bio-tech Co., Ltd, Shanghai GeneoDx Biotech Co., Ltd, DAAN Gene Co., Ltd of Sun Yat-sen University, Sansure Biotech Inc., and Shanghai BioGerm Medical Co., Ltd. The different target genes ( Table 1 ) produced typical S-shaped amplification curves, indicating that the RNA could be used in the six kits to evaluate their LoDs. The viral RNA concentration was determined with RT-droplet digital PCR (RT-ddPCR), which allows the absolute quantification of viral RNA by counting single molecules, without reference to an external standard curve. Correlation of chest CT and RT-PCR testing in coronavirus disease 2019 (COVID-19) in China: A report of 1014 cases Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR We acknowledge the six manufacturers for providing the SARS-CoV-2 RT-PCR detection kits.	./cache/cord-279476-h7zi82a8.txt	./txt/cord-279476-h7zi82a8.txt