id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279439-h4ji0ttm	Viotti, Manuel	HUMAN PRE-IMPLANTATION EMBRYOS ARE PERMISSIVE TO SARS-COV-2 ENTRY	2020-09-30		.txt	text/plain	517	41	49	DESIGN: Assessment of expression levels of SARS-CoV-2 entry mediators in human embryo biopsies by RNAseq analysis, and infection of cultured embryos with SARS-CoV-2 Spike glycoprotein pseudotyped reporter virions expressing green fluorescent protein (GFP). MATERIALS AND METHODS: Trophectoderm biopsies from blastocyst-stage embryos (n¼24) were processed for RNAseq using a commercial kit and sequenced; results were analyzed for expression of factors implicated in SARS-CoV-2 cellular entry. For viral infection experiments, blastocyst-stage embryos (n¼94) were hatched from zonas mechanically, and infected by spinoculation with GFP-reporter virions pseudotyped with the SARS-CoV-2 Spike glycoprotein (required for SARS-CoV-2 entry). RESULTS: Cells collected from blastocyst-stage embryos robustly expressed the canonical SARS-CoV-2 entry receptor ACE2 and the putative activator protease TMPRSS2, in addition to other reported entry factors. Embryos exposed to reporter virions pseudotyped with SARS-CoV-2 Spike glycoprotein displayed robust GFP signal, often in numerous cells with cytoplasmic localization. CONCLUSIONS: Our results indicate that cells present in preimplantation embryos are permissive to the canonical Spike-ACE2 viral entry mechanism utilized by SARS-CoV-2.	./cache/cord-279439-h4ji0ttm.txt	./txt/cord-279439-h4ji0ttm.txt