id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-278678-ivye1qao	Calvez, R. M.	Molecular detection of SARS-CoV-2 using a reagent-free approach	2020-05-02		.txt	text/plain	3223	186	55	Optimisation of the heat-treatment method and inhibitory properties of UTM In a preliminary study to evaluate the best assay conditions, a range of SARS-CoV-2-positive and negative samples was selected and heat-treated at different temperatures and for different times. Each heat-treated sample was then subject to SARS-CoV-2 testing using our in-house assay derived from the CDC resource website (targeting the N-gene and using the TaqMan® Fast Virus 1-Step RT-qPCR kit from ABI). As shown in table 2 below, the ABI TaqMan® One-Step RT-qPCR mix systematically failed to detect SARS-CoV-2 in swab samples resuspended in UTM (COPAN swabs) even in the presence of viral loads greater than 1×10 6 copies/mL (003850 and 003862). Although heat-treatment of respiratory samples prior to RT-qPCR showed an attractive methodology compared to a conventional nucleic acid extraction method, the addition of an internal control is critical for quality control and successful detection of this virus if present in the patient samples.	./cache/cord-278678-ivye1qao.txt	./txt/cord-278678-ivye1qao.txt