id author title date pages extension mime words sentences flesch summary cache txt cord-277874-cr53ycrm Neault, N. SARS-CoV-2 Protein in Wastewater Mirrors COVID-19 Prevalence. 2020-09-03 .txt text/plain 7079 372 49 We believe MPAD based SARS-CoV-2 protein quantitation represents a promising epidemiological tool with a sensitivity sufficiently superior to viral RNA measurement that, in addition to enabling early detection and population tracking of COVID-19 load, will also open the way to effective infection surveillance of specific facilities, schools and residences. Primary sludge and PEG precipitated influent fractions, collected from the contiguous cities of Ottawa and Gatineau in April through June 2020, were analysed for the presence of four SARS-CoV-2 structural proteins, N (nucleocapsid), M (membrane), S (spike), and E (envelope), by western blot. Next, in order to assure specificity for detection of SARS-CoV-2 proteins, we used MPAD with an expanded panel to simultaneously measure three viral proteins, N, S and M, along with six fecal content control proteins in PEG precipitated "influent solids" samples drawn from the Ottawa WRRF during the study period ( Fig 5) . ./cache/cord-277874-cr53ycrm.txt ./txt/cord-277874-cr53ycrm.txt