id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276034-a8pixbuc	Zhi, Yan	Identification of murine CD8 T cell epitopes in codon-optimized SARS-associated coronavirus spike protein	2005-04-25		.txt	text/plain	6210	298	49	Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding codon-optimized SARS-CoV spike protein. The optimized recombinant adenoviral vaccine vectors encoding spike can generate robust antigen-specific cellular immunity in mice and may potentially be useful for control of SARS-CoV infection. Therefore, several versions of replication-defective adenoviral vectors expressing spike protein were created to induce spike-specific T cell responses in mice and to screen for CD8 T-cell epitopes using an overlapping peptide library spanning the entire spike protein in IFN-g ELISPOT and intracellular IFN-g staining assays. More importantly, a single administration of the optimized SARS-CoV spike vaccine vectors based on replication-defective human and simian adenovirus can generate strong spikespecific CD8 T-cell responses in mice. More importantly, a single administration of an optimized SARS-CoV spike vaccine vector based on a replication-defective simian adenovirus can generate strong spike-specific CD8 T-cell responses in mice.	./cache/cord-276034-a8pixbuc.txt	./txt/cord-276034-a8pixbuc.txt