id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-275746-3sgbpn13	Shimamoto, Yasuhiro	Fused-ring structure of decahydroisoquinolin as a novel scaffold for SARS 3CL protease inhibitors	2015-02-15		.txt	text/plain	6600	347	66	After the reaction mixture was cooled to room temperature, water was added and the whole was extracted with AcOEt. The organic layer was washed with 1 M HCl and brine, dried over MgSO 4 , filtered, and concentrated. After the mixture was stirred for 12 h, the reaction was quenched with saturated aqueous NH 4 Cl and the whole was extracted with AcOEt. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered, and concentrated. The residue was purified by silica gel column chromatography (hexane/AcOEt = 3:1) to give a title alcohol ( 21 .0 mmol) in CH 2 Cl 2 (50 mL), and the mixture was stirred for 8 h at room temperature. The residue was purified by silica gel column chromatography (hexane/AcOEt = 6:1) to give 32 ( Tris-HCl buffer pH 7.5 containing 7 mM DTT) was incubated with the R188I SARS 3CL pro28 (56 nM) at 37°C for 60 min in the presence of various inhibitor concentrations at 37°C for 60 min.	./cache/cord-275746-3sgbpn13.txt	./txt/cord-275746-3sgbpn13.txt