id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-275482-ncrhb75f	Jia, Hong Peng	Infection of Human Airway Epithelia by Sars Coronavirus is Associated with ACE2 Expression and Localization	2006		.txt	text/plain	2141	110	42	In contrast with results in polarized epithelia, poorly differentiated primary human tracheobronchial epithelia or A549 cells grown on tissue culture plastic expressed little ACE2 mRNA or protein. (D) β-galactosidase levels determined in primary human airway epithelia cultured under ALI or resubmerged conditions that were infected from the apical with SARS-S protein pseudotyped FIV. These results indicated that SARS-CoV infects undifferentiated human airway epithelial cells poorly or not at all, while well-differentiated conduction airway epithelia are susceptible. Our studies revealed the novel observation that SARS-CoV infection of human airway epithelia is dependent upon the state of epithelial differentiation and ACE2 mRNA and protein expression. In conclusion, studies in models of human airway epithelial differentiation and polarity reveal that SARS-CoV infects well-differentiated cells from the apical surface and preferentially exits from the apical side.	./cache/cord-275482-ncrhb75f.txt	./txt/cord-275482-ncrhb75f.txt