id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-275357-yx8lsfdv	Lu, J.	Saliva is less sensitive than nasopharyngeal swabs for COVID-19 detection in the community setting	2020-05-15		.txt	text/plain	2348	130	56	The Limits of Detection of two sets of RT-PCR assays, the TaqPath Multiplex RT-PCR COVID-19 Kit (Thermo) and the PrimerDesign COVID-19 assay, were determined using different viral RNA and reaction volumes ( Supplementary Table 1 ). In the Helix lab, a miniaturized 5 uL input RNA (10 uL total reaction volume) TaqPath assay was performed on a Quantstudio 7 qRT-PCR instrument (Thermo), and the limit of detection was determined to be 6.25 viral copies. In the UCSD lab, a miniaturized 2 uL input RNA (3 uL total reaction volume) TaqPath assay was performed on a Quantstudio 5 qRT-PCR instrument (Thermo), and the limit of detection was determined to be 3.125 viral copies. RNA was extracted from the NPS VTM and Saliva samples and analyzed using the PrimerDesign and TaqPath assays at the sites shown in Table 2 (full dataset with Ct values for each viral target sequence and internal control sequences are shown in Supplementary Table 2 ).	./cache/cord-275357-yx8lsfdv.txt	./txt/cord-275357-yx8lsfdv.txt