id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274205-e2r38v29	Tsunetsugu-Yokota, Yasuko	Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen	2007-11-28		.txt	text/plain	2170	165	66	title: Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen In general, a whole virion serves as a simple vaccine antigen and often essential material for the analysis of immune responses against virus infection. In order to develop an effective vaccine and diagnostic tools, we prepared UV-inactivated SARS coronavirus on a large scale under the strict Biosafety Level 3 (BSL3) regulation. We have demonstrated that subcutaneously administered UV-inactivated SARS-CoV elicits a high level of IgG-type neutralizing antibodies and weak T-cell responses in mice (4). Here we describe our protocol for the largescale preparation of UV-inactivated SARS-CoV virion under the strict Biosafety Level 3 (BSL3) regulation. In order to increase the safety of this UV-inactivated SARS-CoV, we inactivated the virion vaccine using both UV and formalin. Severe acute respiratory syndrome (SARS) coronavirus: application of monoclonal antibodies and development of an effective vaccine	./cache/cord-274205-e2r38v29.txt	./txt/cord-274205-e2r38v29.txt