id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273784-sr6afv60	Cazares, Lisa H.	Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein	2020-09-23		.txt	text/plain	5793	299	54	The assay was evaluated using mock test samples containing inactivated SARS-CoV-2 virions, added to in vitro derived mucus. To determine the feasibility of targeted MS for SARS-CoV-2 diagnostics, we developed a method for the detection and quantitation of the S and NP, which employs parallel reaction monitoring (PRM) using a high-resolution Orbitrap instrument, thereby providing very high specificity. To test the ability of the PRM assay to detect S protein and NP in a relevant sample type, we spiked inactivated SARS CoV-2 virions into in vitro derived mucus. (B) PRM assay was then used to quantitate the SARS-CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in vitro derived mucus. Using the calibration curve from the best performing peptide for NP (DQVILLNK), the low and high SARS-CoV-2 mock samples contained on average 227 and 422 amol of NP, respectively, on column (see Figure 5A ).	./cache/cord-273784-sr6afv60.txt	./txt/cord-273784-sr6afv60.txt