id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270788-w0pewq52	Chou, Chih-Fong	A novel cell-based binding assay system reconstituting interaction between SARS-CoV S protein and its cellular receptor	2004-11-05		.txt	text/plain	4759	241	64	A binding epitope with lesser degree of glycosylation and native conformation was localized by using rabbit anti-sera raised against five denatured recombinant S protein fragments expressed in Escherichia coli. Cells were washed once with PBS and three times with PBS containing 500 mM NaCl. To test whether the anti-sera from SARS patients or raised against S protein fragments can block the binding between CHO-SG and Vero E6, dislodged CHO-SG cells were preincubated with the anti-serum to be tested at 4 • C for 1 h. The CHO-G cells resisted the PBS wash but were detached by the solutions containing supplemented NaCl. In contrast, the binding between CHO-SG and Vero E6 could resist up to 1M NaCl. We sought to investigate the binding specificity of this interaction by testing whether the serum from a recovered SARS patient (P8 in Tan et al., 2004a ) and a goat anti-ACE2 antibody could block the interaction.	./cache/cord-270788-w0pewq52.txt	./txt/cord-270788-w0pewq52.txt