id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269465-3fdjqnhb	Leth-Larsen, Rikke	The SARS coronavirus spike glycoprotein is selectively recognized by lung surfactant protein D and activates macrophages	2007-05-15		.txt	text/plain	7168	404	61	The severe acute respiratory syndrome coronavirus (SARS-CoV) infects host cells with its surface glycosylated spike-protein (S-protein). Macrophages, DCs, 293T and Vero cells were harvested, incubated for 30 min with 20% (v/v) goat serum, and then stained for 45 min on ice with a mouse monoclonal anti-ACE2 antibody. Cells were washed twice with the binding buffer and then incubated with the myc antibody followed by goat anti-mouse IgG and analyzed by flow cytometry as above. To examine whether S-protein expressed in this study is recognized by ACE2, Vero cells were incubated with the purified S-protein and bound S-protein was detected by flow cytometry using a myc antibody. Blocking of Binding of S-protein to macrophages and DCs: (A) macrophages and DCs were cultured from monocytes and, upon washing, incubated with a mouse anti-human ACE2 monoclonal antibody (solid lines) or, as a control, isotype IgG (solid histograms).	./cache/cord-269465-3fdjqnhb.txt	./txt/cord-269465-3fdjqnhb.txt