id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269025-2j37561h	Pratelli, Annamaria	Canine coronavirus inactivation with physical and chemical agents	2007-05-21		.txt	text/plain	6155	325	53	The methods for CCoV inactivation could be applied as animal models to study human coronavirus infection, reducing the risk of accidental exposure of researchers to pathogens during routine laboratory procedures. To examine the ability of heat to inactivate CCoV, 500 lL aliquots of virus samples were incubated in duplicate in a 15 mL polypropylene conical tube (Falcon, Becton Dickinson Labware) for increasing periods of time at three different temperatures: +56°C, +65°C and +75°C. To examine formaldehyde and glutaraldehyde inactivation of CCoV, aliquots of virus and aldehyde samples, each at two different dilutions, were incubated at +4°C, +25°C and +37°C, respectively, for up to 3 days. Despite the differences in stability during heat treatment at 56°C between SARS-CoV and CCoV, the several methods of CCoV inactivation, including inhibition of viral entry, could be applied as animal models to study human coronavirus infection, reducing the risk of accidental exposure to the virus through unsafe laboratory practices.	./cache/cord-269025-2j37561h.txt	./txt/cord-269025-2j37561h.txt