id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267960-r5m7o9dp	Hourdel, Véronique	Rapid Genomic Characterization of SARS-CoV-2 by Direct Amplicon-Based Sequencing Through Comparison of MinION and Illumina iSeq100(TM) System	2020-09-25		.txt	text/plain	4466	211	51	In this study, we aimed at implementing an ampliconbased sequencing approach to obtain SARS-CoV-2 consensus genomes directly from clinical specimens, adaptable into the field conditions, with the two easily manageable nextgeneration sequencers, the nanopore MinION and the Illumina iSeq100 TM system. Sputum specimens and respective isolates RNA extracts were tested with the SARS-CoV-2 real-time RdRP gene duplex reverse transcription (RT)-PCR developed by the French National Reference Center for Respiratory Viruses and the real-time E gene RT-PCR from the Charité protocol (see WHO Coronavirus disease COVID-19 technical guidance: Laboratory testing for 2019-nCoV in humans, available from https://www.who. Globally, using our amplicon-based approach, combined with the MinION platform, we were able to obtain the near fulllength genome of the studied viral specimens in around 8 h, from samples to sequences data. Viral consensus genomic sequences were rapidly and easily obtained for the two SARS-CoV-2 clinical specimens and their respective isolates, by using the two different sequencing platforms, MinION and iSeq100 TM system.	./cache/cord-267960-r5m7o9dp.txt	./txt/cord-267960-r5m7o9dp.txt