id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267246-hq7g62p5	Huang, Su-Hua	Phage display technique identifies the interaction of severe acute respiratory syndrome coronavirus open reading frame 6 protein with nuclear pore complex interacting protein NPIPB3 in modulating Type I interferon antagonism	2015-07-31		.txt	text/plain	3422	190	41	title: Phage display technique identifies the interaction of severe acute respiratory syndrome coronavirus open reading frame 6 protein with nuclear pore complex interacting protein NPIPB3 in modulating Type I interferon antagonism METHODS: This study identified SARS-CoV ORF6-interacting proteins using the phage displayed human lung cDNA libraries, and examined the association of ORF6–host factor interaction with Type I IFN antagonism. RESULTS: The highest affinity clone to ORF6 displayed the C-terminal domain of NPIPB3 (nuclear pore complex interacting protein family, member B3; also named as phosphatidylinositol-3-kinase-related kinase SMG-1 isoform 1 homolog). The nucleotide sequences of the C terminus (amino acid residues 936e1050) of NPIPB3 (Accession Number Q92617) fused with the coat protein of ORF6-interacting phage clone 40 was amplified using PCR, and then cloned into bacterial expression vector pET32a for coimmunoprecipitation in vitro and mammalian expression vector pDsRed1-C (BD Biosciences Clontech) for colocalization assay.	./cache/cord-267246-hq7g62p5.txt	./txt/cord-267246-hq7g62p5.txt