id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266903-lxtxqdst	Lee, Jong-Hwan	A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)	2020-10-15		.txt	text/plain	2514	147	62	In this study, we designed and developed a novel rapid detection method for SARS-CoV-2 spike 1 (S1) protein using the SARS-CoV-2 receptor ACE2, which can form matched pairs with commercially available antibodies. ACE2 and S1-mAb were paired with each other for capture and detection in a lateral flow immunoassay (LFIA) that did not cross-react with SARS-CoV Spike 1 or MERS-CoV Spike 1 protein. To decrease the non-specific interaction between capture probes in test lines and 20 detection probes, the nitrocellulose membrane was treated with the blocking solution (10 mM 2-21 amino-2-methyl-1-propanol (pH 9.0), 0.5% BSA, 0.5% β-Lactose, 0.05% Triton X-100, 0.05% 22 sodium azide) for 1 hour in a vacuum oven (37°C). -The human ACE2 and commercial antibody were paired with each other as capture and detection probes in a lateral flow immunoassay that was not cross-reactive with SARS-CoV S1 and MERS-CoV S1 proteins.	./cache/cord-266903-lxtxqdst.txt	./txt/cord-266903-lxtxqdst.txt