id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265228-afbkp3wm	Fomsgaard, Anna S.	An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020	2020-04-09		.txt	text/plain	1797	103	57	The comparison of the SensiFAST Probe No-ROX One-Step Real-time PCR results using the simplified workflow to both NA purification systems is shown in Table 1 , Table 2 Comparison of results obtained with the SensiFAST Probe No-ROX One-Step Real-time PCR a assay on clinical samples, which were prior subjected to various minimal processing methods or nucleic acid extractions b , Denmark, 2020 (n = 87 patient samples c ) SARS-CoV-2 positive and negative oropharyngeal swab-samples heat-processed for 5 min at 98 °C before the RT-qPCR reaction showed a 97.4% sensitivity, 100% specificity and 98.3% accuracy compared with MagNA Pure 96 purified samples when using the SensiFAST assay ( Table 1 ). Due to the alarmingly low accessibility to NA purification reagents and kits, we show an alternative to the MagNA Pure purification step with simple heating for 5 min at 98 °C that results in a sensitivity, specificity, and accuracy of 97.4% (95% CI: 86.2-99.9), 100.0% (95% CI: 84.6-100.0) and 98.3% (95% CI: 91.1-99.9), respectively, using the SensiFAST SARS-CoV-2 RT-qPCR assay (Supplementary Data).	./cache/cord-265228-afbkp3wm.txt	./txt/cord-265228-afbkp3wm.txt