id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263039-uoxaem82	Perchetti, Garrett A.	Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection	2020-07-23		.txt	text/plain	664	42	58	Nucleic acid degradation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can compromise the accuracy of molecular detection methods. It has been demonstrated that nasopharyngeal specimens containing SARS-CoV-2 can be stored in phosphate-buffered saline (PBS) as a substitute for viral transport medium (VTM) for up to 7 days (3). Here, we evaluate the stability of differing viral loads of SARS-CoV-2 over 28 days stored at room temperature, 4°C, -20°C, or -80°C. For the high concentration of SARS-CoV-2, regardless of storage conditions, 100% of samples were detected by qRT-PCR through day 28. For lower concentrations of virus, storage at room temperature was associated with reductions of positivity beginning at day 7, and by day 28, 0% of samples were detected for N1. At viral loads of Ͼ5,000 copies/ ml-corresponding to Ͼ75% of positive samples recovered in our clinical lab to date-different storage temperatures did not have a substantial impact on our ability to detect SARS-CoV-2 when stored in PBS.	./cache/cord-263039-uoxaem82.txt	./txt/cord-263039-uoxaem82.txt