id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262250-o7qhncic	Habel, J. R.	Suboptimal SARS-CoV-2-specific CD8+ T-cell response associated with the prominent HLA-A*02:01 phenotype	2020-08-19		.txt	text/plain	6206	308	57	Using peptide-HLA-I tetramers, we performed direct ex vivo tetramer enrichment to define the frequency and activation profiles of the responding SARS-CoV2-specific CD8 + T-cells in acute and convalescent COVID-19 patients and in prepandemic PBMCs, tonsil and lung tissues from uninfected donors. 17.20176370 doi: medRxiv preprint To further probe the the responsiveness of A2/SARS-CoV-2 CD8 + T-cells from uninfected versus convalescent COVID-19 donors, PBMCs or tonsil cells were stimulated with the S 269 and Orf1ab 3183 peptides and cultured in vitro for 10 days. Our findings show that, while 'early memory' CD8 + T-cells can be detected in convalescent HLA-A*02:01 COVID-19 patients at frequencies ∼5-fold higher than those from pre-pandemic samples, the SARS-CoV-2-specific response was ∼10-fold lower than that found regularly for CD8 + T-cells directed at IAV or EBV epitopes. Even so, it is the case that SARS-CoV-2-specific CD8 + T-cells were found in all COVID-19 acute and convalescent donors, and in stored pre-pandemic PBMC and tonsil samples (but not lung tissues) from HLA-A*02:01 children, mature adults and the elderly.	./cache/cord-262250-o7qhncic.txt	./txt/cord-262250-o7qhncic.txt