id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261414-vqvctafm	Ian Gallicano, G.	Molecular targeting of vulnerable RNA sequences in SARS CoV-2: identifying clinical feasibility	2020-11-12		.txt	text/plain	3032	230	58	Here we show that siRNAs and miRNAs inhibit SARS CoV-2 spike protein production in a dose-dependent manner in both HEK293 cells and a primary human airway tracheal cell line. Two cell types, HEK293 cells and primary human tracheal cells (hpTCs) were employed to test the hypothesis that siRNAs or miRNAs could suppress SARS CoV-2 spike expression. As a result, electroporation was used to introduce spike cDNA into hpTCs. Twenty four hours after electroporation of spike plasmid, 200 nM of each small RNA (the concentration seen to virtually completely suppress spike in HEK293 cells) was transfected resulting in marked reduction in spike protein expression in both siRNA1 + 2 and miRNA1 + 2 samples (Fig. 3A) . The data in Fig. 3B , C reveal that siRNA1/NT (non-transfection reagent) suppressed spike protein production in a dose-dependent manner in HEK293 cells (Fig. 3B ).	./cache/cord-261414-vqvctafm.txt	./txt/cord-261414-vqvctafm.txt